Expression of Recombinant Coat Protein (CP) of Citrus tristeza virus for Polyclonal Antibodies Production
نویسندگان
چکیده
منابع مشابه
Prokaryotic Expression, Purification, and Polyclonal Antibody Production of a Truncated Recombinant Rabies Virus L Protein
Background: Rabies virus (RABV) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. L protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication. Objectives: A truncated L protein of Rabies virus is being cloned, expressed and purified to produce relevant...
متن کاملexpression of citrus tristeza virus coat protein gene in escherichia coli
citrus tristeza virus (ctv) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in iran, as one of the major citr...
متن کاملExpression of Citrus tristeza virus coat protein gene in Escherichia coli
Citrus tristeza virus (CTV) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. Considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in Iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in Iran, as one of the major citr...
متن کاملBacterial expressed coat protein: development of a single antiserum for routine detection of Citrus Tristeza Virus
Citrus tristeza virus (CTV, genus Closterovirus, family Closteroviridae) is the largest member of its taxonomic family. Viral particles, 2000 nm in length and 10–12 nm in diameter, are present in the phloem of infected plants. A single stranded positive sense RNA molecule approximately 20,000 nucleotides long constitutes its genome (BarBacterial expressed coat protein: development of a single a...
متن کاملProduction and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis
Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were characterized for their immunoglobulin isotype and their titres in cell culture and ascites fluids. Each MAb was conjugated with alkaline phosphatase and...
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ژورنال
عنوان ژورنال: Asian Journal of Agricultural Research
سال: 2014
ISSN: 1819-1894
DOI: 10.3923/ajar.2014.114.121